fluorescent dye some introductory

A fluorescent dye is a dye , such as certain types of vitamins (A, B2), biological pigments, lipids , elastic fiber, etc. autofluorescence under the microscope and imaging effects color effects are very weak, but significant as the time is short . Some organisms in the shortwave spectrum chemical composition under irradiation spontaneously excited fluorescence , called autofluorescence (Primaryfluorescence). Such dibasic lead stearate  as certain types of vitamins (A, B2), biological pigments, lipids , elastic fiber, etc. autofluorescence under the microscope and imaging effects color effects are very weak , and the imaging time is short . Biological specimens treated with a fluorescent dye after irradiation in the shortwave spectrum can inspire brilliant fluorescence , called secondary fluorescence, secondary fluorescence imaging effect color effects and very strong . So it occupies in fluorescence microscopy techniques most important position. Today synthesized in fluorescence microscopy techniques more efficient fluorescent dye , to develop more specific markers , fluorescent dye and the excitation wavelength of the variable relationship between the fluorescence intensity and quantitative determination of chromosome staining fluorescence scanning , etc. are classified material exploring the direction
Fluorescent pigments can be divided into three general categories .
1 fluorescent dye such alkaline  plastic additive dye in an acidic solution easily dissociate . The fluorescence component with a positive charge . So that is able and biological specimens acidic ion- binding group . In alkaline solution in a non- dissociated state.
2 Acid fluorescent dye in alkaline solution easily dissociated components of the fluorescence with a negative electrical charge.
3 fluorescent dye acidic neutral or weak alkaline electrically neutral dye fluorescent dye in the staining process of dissociation no practical significance. This pigment is used for display macromolecular structures .
Using a fluorescent dye vast areas, particularly for example mineral oil in mineralogy identified impurities , optical glass industry classification identified in quartz sand , hydrogeology identified magnesium hydroxide  reservoir leakage , forensic detection of blood, semen stains , mechanical industry to check metal cracks , classical art and fake identification, bacteriology , virology , immunology , oncology , genetics, fiber textile industry and various other specialized fluorescence techniques can be applied .